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J Virol. 1973 July; 12(1): 13-17
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Isolation and Properties of Bacillus subtilis Strains Lysogenized by a Clear Plaque Mutant of Bacteriophage 105

¹ Department of Microbiology, Mount Sinai School of Medicine, The City University of New York, New York, New York 10029

ABSTRACT

A clear plaque mutant of the temperate Bacillus phage 105 lysogenized a small fraction of infected cells forming an integrated prophage at or near the normal 105 insertion site. These lysogens exhibited a spontaneous induction rate approximately 1,000-fold lower than wild type and were noninducible (ind^-) by mitomycin C. Prophage was induced, however, when competent cultures were incubated with transforming DNA. The ind^- phenotype could not be attributed solely to the clear plaque mutation and appears to involve a cell-specific factor. Lysogenization by the clear plaque mutant, in contrast to wild-type phage, did not cause a marked reduction in transformation efficiency.