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J Virol. 1973 June; 11(6): 991-997
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Growth Characteristics of Cytomegalovirus in Human Fibroblasts with Demonstration of Protein Synthesis Early in Viral Replication

^a The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104

ABSTRACT

In high-multiplicity infection of human fibroblasts, human cytomegalovirus of WI-38 human diploid cells produced early cell rounding 6 to 24 h after inoculation. This early cell rounding was caused only by inoculation with infectious virions. Inhibitors of protein synthesis, but not DNA inhibitors, prevented this cytopathic effect. Apparently, a new protein is synthesized in infected fibroblasts from about 2 h postinoculation. Infectivity of cell-associated and supernatant infectious virus reached maximal levels at 5 to 7 and 10 days postinoculation, respectively. Synthesis of DNA, infectious virus, complement-fixing antigen, and precipitin antigen all began between 24 and 48 h, with the bulk of synthesis occurring 48 to 96 h postinoculation.

¹ Permanent address: Farmitalia Research Laboratory, Milan, Italy.

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