This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Heine, J. W. Articles by Roizman, B. Search for Related Content PubMed PubMed Citation Articles by Heine, J. W. Articles by Roizman, B.

 Previous Article  |  Next Article 

J Virol. 1973 May; 11(5): 810-813
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Proteins Specified by Herpes Simplex Virus IX. Contiguity of Host and Viral Proteins in the Plasma Membrane of Infected Cells

Department of Microbiology, The University of Chicago, Chicago, Illinois 60637

ABSTRACT

Artificial mixtures of plasma membrane vesicles produced by microcavitation from infected and uninfected cells band at the same density on isopycnic centrifugation in sucrose density gradient. However, after reaction with antiviral antibody, the density of the infected cell plasma membrane vesicles increases, and the infected and uninfected cell membranes are quantitatively separable on isopycnic centrifugation. Plasma membrane vesicles prepared from cells doubly labeled before and after infection with radioactive amino acids and reacted with antibody banded at a high density. Polyacrylamide gel electropherograms show that the vesicles reacted with antibody consist of both host- and virus-specific membrane proteins. Microcavitation does not disrupt viral envelopes since infectivity is not affected by this procedure. We conclude that viral and cellular proteins in the plasma membrane preparations are contiguous.