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J Virol. 1973 May; 11(5): 665-671
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Studies of Nondefective Adenovirus 2-Simian Virus 40 Hybrid Viruses. VI. Characterization of the DNA from Five Nondefective Hybrid Viruses

Patrick H. Henry, Lowell E. Schnipper, Richard J. Samaha, Clyde S. Crumpacker1, Andrew M. Lewis Jr. and Arthur S. Levine

Division of Hematology-Oncology, Department of Medicine, University of Missouri Medical Center, Columbia, Missouri 65201
Hematology Branch, National Cancer Institute, Bethesda, Maryland 20014
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland 20014

ABSTRACT

Certain biophysical characteristics of the DNA from each of the five nondefective adenovirus 2 (Ad2)-simian virus 40 (SV40) hybrid viruses (Ad2+ND1, Ad2+ND2, Ad2+ND3, Ad2+ND4, Ad2+ND5) have been determined. The guanine plus cytosine content varied from 55 to 57% and was not significantly different from that of nonhybrid Ad2 (56%), and the hybrid DNA molecules had mean molecular lengths which were similar to that of the standard, Ad2. The Ad2 and SV40 components of each hybrid were linked by alkali-resistant, presumably covalent bonds. The percentage of SV40 DNA in each hybrid virus was determined by hybridization with SV40 complementary RNA in a calibrated system. The results indicate that each hybrid virus DNA contains a different percentage of SV40 nucleotide sequences. The estimated size of the SV40 DNA component varies from 48,000 daltons for Ad2+ND3 to 840,000 daltons for Ad2+ND4, the latter being equivalent to between one-fourth and one-third of the SV40 genome.

FOOTNOTES

1 Present address: Channing Laboratory, Boston City Hospital, Boston, Mass. 02118.

J Virol. 1973 May; 11(5): 665-671
Copyright © 1973 American Society for Microbiology. All Rights Reserved.





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Copyright © 1973 by the American Society for Microbiology. All rights reserved.