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J Virol. 1973 May; 11(5): 621-629
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Growth of Coliphage T7 in Salmonella typhimurium

^a Department of Microbiology and Immunology, Duke University Medical Center, Durham, North Carolina 27710

ABSTRACT

A mutant of Salmonella typhimurium was found to be sensitive to killing by coliphage T7 because of an alteration in its surface properties. However, the infections were abortive and studies with ³²P-labeled T7 grown in Escherichia coli B (T7·B) indicated that the phage DNA was restricted by S. typhimurium. When a mutant T7 which survived the restriction and produced plaques on Salmonella (T7·S) was passed through one cycle of growth in E. coli B, its ability to grow in Salmonella was lost, indicating that host-controlled restriction and modification are operative in this system. Restrictionless S. typhimurium mutants were isolated that permit the growth of not only T7·S but also T7·B and coliphage T3. The physiology of T7 production in the restrictionless host is nearly identical to that in Escherichia coli.

¹ Present address: Radiobiology Laboratories, Yale University School of Medicine, New Haven, Conn. 06510.