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J Virol. 1973 May; 11(5): 615-620
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Molecular Weight Determination of Sendai RNA by Dimethyl Sulfoxide Gradient Sedimentation

Department of Molecular Biology, University of Geneva, Geneva, Switzerland

ABSTRACT

The molecular weight of the large RNA of Sendai virus has been determined by sedimentation analysis in sucrose gradients containing 99% dimethyl sulfoxide (DMSO) to be 2.3 x 10⁶. Sendai RNA recovered from 99% DMSO was found to cosediment with nondenatured Sendai RNA at 46 to 48s in ordinary sucrose gradients. The molecular weight value of 2.3 x 10⁶ is considerably smaller than the estimates of 6 x 10⁶ to 7 x 10⁶ determined under nondenaturing conditions, suggesting a unique structure for Sendai RNA.