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J Virol. 1973 February; 11(2): 329-334
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Structural Organization of Murine Intracisternal A Particles

¹ Laboratories of Cell Biology and Biochemistry, National Cancer Institute, Bethesda, Maryland 20014

ABSTRACT

Isolated murine intracisternal A particles have a distinctive set of structural properties as compared to the recognized oncogenic ribonucleic acid tumor viruses. A particles consist of two closely approximated concentric shells. The outer shell is largely, but not entirely, susceptible to treatment with detergents such as deoxycholate and Triton X-100. The inner shell is resistant to these agents and is stabilized against solubilization by sodium dodecyl sulfate as a result of disulfide bonding between its constituent proteins. The major A particle structural protein (molecular weight approximately 70,000) is contained in the inner shell. A protein component with a molecular weight of about 80,000 may be preferentially concentrated in sites of firm attachment between the inner and outer shells.