This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Stone, H. O. Articles by Kingsbury, D. W. Search for Related Content PubMed PubMed Citation Articles by Stone, H. O. Articles by Kingsbury, D. W.

 Previous Article  |  Next Article 

J Virol. 1973 February; 11(2): 243-249
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Stimulation of Sendai Virion Transcriptase by Polyanions

¹ Laboratories of Virology and Immunology, St. Jude Children's Research Hospital, and The University of Tennessee Medical Units, Memphis, Tennessee 38101

ABSTRACT

Exogenous polyribonucleotides stimulated the ribonucleic acid (RNA) transcriptase in Sendai virions. Added yeast RNA, polyadenylic acid, or polycytidylic acid increased incorporation of ³H-guanosine monophosphate as much as fivefold. The products of stimulated reactions were virus-specific as determined by hybridization with Sendai virion RNA, but they sedimented more slowly (13s) than the product of an unstimulated reaction (16s). The stimulating activity was nondialyzable and heat stable, but was abolished by alkaline hydrolysis. Nucleoside monophosphates, individually or in combination, were ineffective, confirming the requirement for a polymer. Among other substances tested for effects on Sendai virion transcriptase, polyaspartic acid and polyglutamic acid stimulated the enzyme; polyinosinic acid, polyuridylic acid, and polyamines had no effect; and dextran sulfate and polyvinyl sulfate were inhibitory.