This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Loskutoff, D. J. Articles by Pène, J. J. Search for Related Content PubMed PubMed Citation Articles by Loskutoff, D. J. Articles by Pène, J. J.

 Previous Article  |  Next Article 

J Virol. 1973 January; 11(1): 87-97
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Gene Expression During the Development of Bacillus subtilis Bacteriophage 29 II. Resolution of Viral-Specific Ribonucleic Acid Molecules

Department of Molecular, Cellular, and Developmental Biology, University of Colorado, Boulder, Colorado 80302

ABSTRACT

The ribonucleic acid (RNA) specified by bacteriophage 29 was isolated under conditions which minimized physical and enzymatic degradation, reduced aggregation, and enriched for completed molecules. This RNA was fractionated both by sedimentation through sucrose density gradients and electrophoresis through polyacrylamide gels to measure the size and relative amount of each component. Early RNA consisted of six components of molecular weight 0.75 x 10⁶, 0.44 x 10⁶, 0.37 x 10⁶, 0.25 x 10⁶, 0.09 x 10⁶, and 0.04 x 10⁶, accounting for 35% of the coding capacity of 29 deoxyribonucleic acid (DNA). All of these components except the one at 0.44 x 10⁶ were detected when infection occurred in the presence of chloramphenicol. Synthesis of the major early component (0.75 x 10⁶) ceased shortly after the onset of viral DNA synthesis. The other species of early RNA were synthesized throughout the latent period. Three additional components, 1.75 x 10⁶, 0.93 x 10⁶, and 0.07 x 10⁶, appear at late times. The two large RNAs may be polycistronic messenger RNAs corresponding to the seven viral capsid proteins.

This article has been cited by other articles:

• Guo, P., Erickson, S, Anderson, D (1987). A small viral RNA is required for in vitro packaging of bacteriophage phi 29 DNA. Science 236: 690-694 [Abstract]