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J Virol. 1973 January; 11(1): 153-155
Copyright © 1973 American Society for Microbiology. All Rights Reserved.

Selective Replication of Bacteriophage 29 Deoxyribonucleic Acid in 6-(p-Hydroxyphenylazo)-Uracil-Treated Bacillus subtilis

Departments of Biochemistry and Microbiology and School of Dentistry, University of Minnesota, Minneapolis, Minnesota 55455

ABSTRACT

Phage 29 deoxyribonucleic acid (DNA) replicated under conditions where semiconservative DNA production in Bacillus subtilis host cells was blocked with 6-(p-hydroxyphenylazo)-uracil (HPUra). The time of initiation of 29 DNA replication was not affected by HPUra, and normal quantities of viable phage were produced in the presence of the inhibitor. Studies with conditional lethal mutants of phage 29 demonstrated the usefulness of HPUra for detection of viral-specific DNA production.

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