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J Virol. 1972 December; 10(6): 1236-1239
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Density Heterogeneity of Simian Virus 40 Ribonucleic Acid Late After Infection of Permissive Cells

Allan H. Fried

Institut für Virusforschung, Deutsches Krebsforschungszentrum, 69 Heidelberg, Germany

ABSTRACT

Ribonucleic acid (RNA) was isolated from CV-1 cells 44 hr after simian virus 40 (SV40) infection. The molecules containing SV40 base sequences were characterized with respect to their buoyant density distribution. The density of these molecules was compared to that of single- and double-stranded RNA synthesized by using SV40 DNA and Escherichia coli RNA polymerase. The results suggest that about 20% of the SV40 RNA in the infected cells consisted of partially double-stranded molecules.

J Virol. 1972 December; 10(6): 1236-1239
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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J. Bacteriol. Mol. Cell. Biol. Microbiol. Mol. Biol. Rev.
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Copyright © 1972 by the American Society for Microbiology. All rights reserved.