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J Virol. 1972 December; 10(6): 1231-1235
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

The Glycoprotein of Vesicular Stomatitis Virus Is the Antigen That Gives Rise to and Reacts with Neutralizing Antibody

¹ Department of Microbiology, The University of Virginia, School of Medicine, Charlottesville, Virginia 22901

ABSTRACT

The glycoprotein, but no other virion protein, of vesicular stomatitis virus was solubilized by the nonionic detergent Triton X-100 in low ionic strength buffer. The solubilized viral glycoprotein induced the synthesis of antibody that formed a single precipitin line with the glycoprotein and that neutralized the infectivity of the virus. The neutralizing activity of the antibody was efficiently blocked by purified glycoprotein.

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