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J Virol. 1972 December; 10(6): 1220-1227
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Isolation and Characterization of Subacute Sclerosing Panencephalitis Virus Nucleocapsids

¹ The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104

ABSTRACT

Nucleocapsids from subacute sclerosing panencephalitis (SSPE) virus-infected CV-1 cells were concentrated by differential centrifugation employing sucrose cushion techniques and further purified by centrifugation through a linear CsCl density gradient. The bouyant density of ³H-uridine-labeled nucleocapsids in CsCl was found to be 1.31 g/cm³. Ultraviolet absorption spectra of the purified SSPE nucleocapsid showed an absorption maximum at 260 to 265 nm and a 280/260 ratio that corresponded to a nucleic acid content of approximately 4.3%. Negatively stained preparations of SSPE nucleocapsids were found to have a width of 18 ± 1 nm, a periodicity of 5 to 6 nm, and a length between 1-1.4 µm, with the greatest number at 1.3 µm.