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J Virol. 1972 November; 10(5): 959-964
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Serological Analysis of the Deoxyribonucleic Acid Polymerase of Avian Oncornaviruses II. Comparison of Avian Deoxyribonucleic Acid Polymerases

Robert C. Nowinski, K. F. Watson, A. Yaniv and S. Spiegelman

McArdle Laboratory for Cancer Research, University of Wisconsin, Madison, Wisconsin 53706
Institute of Cancer Research, College of Physicians and Surgeons, Columbia University, New York, New York 10032

ABSTRACT

Monospecific antiserum prepared against the isolated deoxyribonucleic acid (DNA) polymerase of avian myeloblastosis virus (AMV) neutralized the endogenous ribonucleic acid-instructed DNA polymerase activity of detergent-disrupted virus. The viral polymerase was serologically unrelated to the seven major structural polypeptides of AMV. Furthermore, the viral enzyme was distinguished from normal cellular DNA polymerases by serological criteria; thus, antiserum against the viral enzyme neutralized its homologous antigen but not normal cellular DNA polymerases. Neutralization by antibody of viral DNA polymerase activity was observed with all avian leukemia-sarcoma viruses tested, irrespective of viral antigenic subtype. The DNA polymerase activity of avian reticuloendotheliosis virus, and of a variety of mammalian oncornaviruses, was not neutralized by antisera against the AMV polymerase. Immunological analysis of the RSV{alpha}(O) mutant, which is deficient in DNA polymerase activity, shows this mutant to lack demonstrable polymerase antigen. Viral polymerase was identified by immunofluorescence as a cytoplasmic constituent in virus-producing chicken cells; polymerase antigen was not detected in uninfected (gs–) chicken cells.

J Virol. 1972 November; 10(5): 959-964
Copyright © 1972 American Society for Microbiology. All Rights Reserved.





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