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J Virol. 1972 November; 10(5): 943-950
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Incorporation of In Vitro Synthesized Reovirus Double-Stranded Ribonucleic Acid into Virus Corelike Particles

S. Sakuma and Y. Watanabe

1 The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania 19104

ABSTRACT

When reovirus double-stranded ribonucleic acid (dsRNA) was synthesized in vitro by using a large-particulate fraction (LP-fraction) from reovirus-infected L cells, a significant amount of the 3H-labeled dsRNA product was incorporated into reovirus corelike particles bound to the LP fraction. These corelike particles were found to be indistinguishable from virus core derived by chymotryptic digestion of virions when compared on the basis of their (i) resistance to chymotryptic digestion, (ii) buoyant density in CsCl, (iii) particle size as determined by agarose chromatography, (iv) elution characteristics from diethylaminoethyl-Sephadex, and (v) resistance of the incorporated 3H-dsRNA to ribonuclease digestion in 0.01 M NaCl. When the replicase reaction was partially inhibited by NaCl, there was an accumulation of particles that were less dense than the virus core. All of the results indicate that some virus core assembly takes place during the in vitro replicase reaction.

J Virol. 1972 November; 10(5): 943-950
Copyright © 1972 American Society for Microbiology. All Rights Reserved.



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