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J Virol. 1972 October; 10(4): 746-750
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Major Group-Specific Protein of Rat Type C Viruses

Flow Laboratories, Inc., Rockville, Maryland 20852
Viral Carcinogenesis Branch, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20014

ABSTRACT

The major internal protein of a rat type C virus pseudotype of murine sarcoma virus, MSV(RaLV), was purified by isoelectric focusing (pI = 8.6) and used to prepare antibody in guinea pigs. The protein was identified by its reaction with antisera reactive with the mammalian type C virus group-specific (gs) antigenic determinant, gs-3. The guinea pig antisera mainly contained species-specific (gs-1) antibody for reactions in gel diffusion with other type C viruses were limited to those of rat origin, whereas in complement fixation tests heterologous reactions could be eliminated by use of appropriate antiserum concentrations without affecting homologous reactions. Guinea pig antisera against mouse, hamster, or cat gs-1 determinants did not react with MSV(RaLV) purified gs protein or with any of several other rat type C viruses.