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J Virol. 1972 October; 10(4): 689-697
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Transcription of the Influenza Ribonucleic Acid Genome by a Virion Polymerase III. Completeness of the Transcription Process

Institute of Microbiology, Rutgers University, The State University of New Jersey, New Brunswick, New Jersey 08903

ABSTRACT

The virion ribonucleic acid (RNA) polymerase of influenza strain A₀/WS transcribed at least 81% of the viral genome in vitro. The polymerase is shown to be associated with each of the major size classes of the virion RNA-ribonucleoprotein complexes. Under optimal in vitro conditions, at least 45% of the RNA contained in a population of influenza virions was involved in a repetitive transcription process. The detectable proteins associated with enzymatically active complexes containing RNA, ribonucleoprotein, and polymerase have been identified by polyacrylamide gel electrophoresis.