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J Virol. 1972 October; 10(4): 639-647
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Isolation and Preliminary Characterization of Temperature-Sensitive Mutants of Influenza Virus

A. Sugiura1, K. Tobita and Edwin D. Kilbourne

a Department of Microbiology, Mount Sinai School of Medicine, The City University of New York, New York, New York 10029

ABSTRACT

Isolation of temperature-sensitive (ts) mutants was attempted from the WSN strain of influenza A virus which was grown and assayed in MDBK cells. After growth of wild-type virus in the presence of 5-fluorouracil, 15 ts mutants were selected for which the ratio of plaquing efficiency at 39.5 C to that at 33 C was 10–3 or less. In pairwise crosses of ts mutants, recombination and complementation were either very efficient or undetectable. It is suggested, therefore, that the viral genome consists of physically discrete units and recombination occurs as an exchange of these units. All 15 mutants have been assigned with certainty into five recombination groups. Three mutants are suspected to be double mutants. Any two complementing mutants always recombined with each other, and noncomplementing mutants did not recombine. In physiological tests, mutants showed diverse patterns of functional defects at the nonpermissive temperature. However, it was not always possible to correlate these physiological defects with the results of genetic characterization.

FOOTNOTES

1 Present address: The Institute of Public Health, Shirokanedai, Minato-ku, Tokyo 108, Japan.

J Virol. 1972 October; 10(4): 639-647
Copyright © 1972 American Society for Microbiology. All Rights Reserved.



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