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J Virol. 1972 July; 10(1): 42-50
Copyright © 1972 American Society for Microbiology. All Rights Reserved.

Specific Fragments of {varphi}X174 Deoxyribonucleic Acid Produced by a Restriction Enzyme from Haemophilus aegyptius, Endonuclease Z 1

June H. Middleton, Marshall H. Edgell and Clyde A. Hutchison III

a Department of Bacteriology and Immunology and Curriculum in Genetics, School of Medicine, University of North Carolina, Chapel Hill, North Carolina 27514

ABSTRACT

A restriction-like enzyme has been purified from Haemophilus aegyptius. This nuclease, endonuclease Z, produces a rapid decrease in the viscosity of native calf thymus and H. influenzae deoxyribonucleic acids (DNA), but does not degrade homologous DNA. The specificity of endonuclease Z is different from that of the similar endonuclease isolated from H. influenzae (endonuclease R). The purified enzyme cleaves the double-stranded replicative form DNA of bacteriophage {varphi}X174 ({varphi}X174 RF DNA) into at least 11 specific limit fragments whose molecular sizes have been estimated by gel electrophoresis. The position of these fragments with respect to the genetic map of {varphi}X174 can be determined by using the genetic assay for small fragments of {varphi}X174 DNA.

FOOTNOTES

1 A preliminary report of this work was presented (Biophys. Soc. Abstr., 1972).

J Virol. 1972 July; 10(1): 42-50
Copyright © 1972 American Society for Microbiology. All Rights Reserved.



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