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J Virol. 1967 December; 1(6): 1227-1237
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

New Assay Procedure for Separation of Mycoplasmas from Virus Pools and Tissue Culture Systems

Izabella Zgorniak-Nowosielska1, W. David Sedwick, Klaus Hummeler2 and Hilary Koprowski

a The Wistar Institute of Anatomy and Biology, The Children's Hospital of Philadelphia, and the World Health Organization International Reference Centre for Rabies at The Wistar Institute, Philadelphia, Pennsylvania 19104

ABSTRACT

Presence of mycoplasma organisms in tissue culture systems and virus pools was detected by titration of the contaminated material on agarose-suspended BHK21/13S cells. The use of this method permitted isolation of mycoplasmas which could not be detected by standard assay methods. Mycoplasma colonies at concentrations ranging from 104 to 106 colony-forming units/ml in agarose-BHK21/13S media could be distinguished from virus plaques, and the two populations of microorganisms could be easily disassociated either by electron microscopy or by biological methods. All isolated mycoplasmas were identified in growth inhibition tests as belonging to the GDL group. The growth inhibition test on agarose-BHK21/13S cell suspension plates could also be applied directly to those strains which could not be isolated by standard assay procedures.

FOOTNOTES

1 Work performed during the tenure of a World Health Organization Senior Research Training grant at The Wistar Institute. Permanent address: Department of Medical Microbiology, Academy of Medicine, Krakow, Poland.

2 Recipient of Public Health Service Award K3-HD-22 708.

J Virol. 1967 December; 1(6): 1227-1237
Copyright © 1967 American Society for Microbiology. All Rights Reserved.





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Copyright © 1967 by the American Society for Microbiology. All rights reserved.