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J Virol. 1967 December; 1(6): 1207-1216
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Purification of Large Quantities of Influenza Virus by Density Gradient Centrifugation

C. B. Reimer1, R. S. Baker, R. M. vanFrank, T. E. Newlin, G. B. Cline2 and N. G. Anderson

Biological Research Division, Lilly Research Laboratories, Indianapolis, Indiana 46205
The Molecular Anatomy Program, Oak Ridge National Laboratory, 3 Oak Ridge, Tennessee 37830

ABSTRACT

New zonal centrifuges can conveniently process as much as five orders of magnitude (105) greater sample volumes than conventional swinging-bucket rotors. The continuous-sample-flow-with-banding versions may be used in series with ancillary purification procedures. Here we have studied the combined process: absorption and elution of influenza virus with barium sulfate followed by concentration and isopycnic banding of the virus in a buffered sucrose gradient. Kilogram quantities of impurity have been rapidly separated from grams of purified virus, which have been conveniently concentrated several hundred-fold by the purification process. Experimental vaccines made by these procedures are being evaluated.

FOOTNOTES

1 Present address: National Communicable Disease Center, Atlanta, Ga. 30333.

2 Present address: Department of Radiology, University of Alabama Medical School, Birmingham, Ala.

3 Operated for the U.S. Atomic Energy Commission by the Nuclear Division of Union Carbide Corp.

J Virol. 1967 December; 1(6): 1207-1216
Copyright © 1967 American Society for Microbiology. All Rights Reserved.



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Copyright © 1967 by the American Society for Microbiology. All rights reserved.