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J Virol. 1967 October; 1(5): 980-987
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Physicochemical Characterization of Adeno-associated Satellite Virus Type 4 and Its Nucleic Acid

Wade P. Parks, Maurice Green1, Magdalena Piña and Joseph L. Melnick

Department of Virology and Epidemiology, Baylor University College of Medicine, Houston, Texas 77025
Institute for Molecular Virology, St. Louis University School of Medicine, St. Louis, Missouri 63110

ABSTRACT

Based on protein, deoxyribonucleic acid (DNA), and phosphorus analyses, adeno-associated satellite virus type 4 has a DNA content of 26.5%, significantly larger than type 1 which contains 18.9% DNA. Type 4 DNA contains 58 to 62% guanine + cytosine, as calculated from thermal denaturation temperature and buoyant density measurements. Simian adenovirus SV15, which serves as a helper virus for type 4 satellite, contains 13.7% DNA with a guanine + cytosine content of 55 to 57%. The DNA extracted from type 4 satellite virus is a linear, double-stranded molecule, as shown by thermal denaturation temperature profile measurements and by electron microscopy. The contour length and sedimentation coefficient of type 4 DNA indicate a molecular weight of 3.0 x 106 daltons.

FOOTNOTES

1 Research career awardee, Institute of Allergy and Infectious Diseases, National Institutes of Health (5-K6-AI4739).

J Virol. 1967 October; 1(5): 980-987
Copyright © 1967 American Society for Microbiology. All Rights Reserved.



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