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Examination of the Qß-Replicase Reaction by Sucrose Gradient and Gel Electrophoresis

^a Department of Microbiology, University of Illinois, Urbana, Illinois 61801

ABSTRACT

The products of an in vitro synthesis with the Qß replicase purified from Escherichia coli infected by the ribonucleic acid (RNA) bacteriophage Qß were examined by sucrose gradient centrifugation and polyacrylamide gel electrophoresis. It was found that, in contrast to sucrose gradients, gel electrophoresis clearly resolved four classes in the newly synthesized RNA. The product was found to contain mature Qß-RNA and small-molecular-weight RNA. In addition, two species were identified which corresponded to the structures found in vivo by Francke and Hofschneider and by Franklin. All of the participants implicated in RNA replication by in vivo studies have now been synthesized in vitro and isolated from the reaction mixture.

¹ Predoctoral trainee in Microbial and Molecular Genetics, Public Health Training Grant 5-T01-GM-319.

² D. H. L. Bishop holds a Wellcome Travel Scholarship.