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J Virol. 1967 August; 1(4): 693-700
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Comparative Studies of the Regeneration of HeLa Cell Receptors for Poliovirus T1 and Coxsackievirus B3 1

Neil H. Levitt and Richard L. Crowell2

a Langbord Virus Laboratory, Department of Microbiology, Hahnemann Medical College, Philadelphia, Pennsylvania 19102

ABSTRACT

Enterovirus receptors of live HeLa cells have been shown to reappear after inactivation by proteolytic enzymes, provided the cells are incubated at 37 C in a nutritionally adequate medium. Regeneration of receptor activity for poliovirus T1 occurred at a significantly faster rate than for coxsackievirus B3. The regenerative process for both types of receptors studied evidently required an active process of protein synthesis, since it was found that reappearance of receptor activity was inhibited by streptovitacin A, puromycin, and actinomycin D. Substitution of p-fluorophenylalanine for the naturally occurring amino acid, at concentrations which inhibited virus synthesis, was without effect on regeneration of receptor activity. It is anticipated that these findings will aid in the study of the biosynthesis and subsequent chemical characterization of viral receptors of living host cells.

FOOTNOTES

2 Public Health Service Research Career Development Award Grantee of the National Institute of Allergy and Infectious Diseases.

1 Presented in part as a preliminary report before the American Society for Microbiology, Los Angeles, Calif., May 1966.

J Virol. 1967 August; 1(4): 693-700
Copyright © 1967 American Society for Microbiology. All Rights Reserved.





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