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Respiratory Syncytial Virus Isolation by Combined Continuous Flow-Isopycnic Banding Centrifugation

Biology Division, Oak Ridge National Laboratory, ¹ Oak Ridge, Tennessee 37830
Laboratory of Infectious Diseases, National Institutes of Health, Bethesda, Maryland 20014
Technical Division, Oak Ridge Gaseous Diffusion Plant, ^,1 Oak Ridge, Tennessee 37830

ABSTRACT

A new zonal centrifuge rotor (B-IX) which combines continuous sample flow centrifugation with isopycnic banding has been used to isolate and concentrate respiratory syncytial virus from liter volumes of culture fluid. This isolation technique utilizes a sucrose density gradient to trap and isopycnically band the virus particles, and permits recovery of the particles from the rotor in an unaggregated condition.

¹ Operated for the U.S. Atomic Energy Commission by the Nuclear Division of Union Carbide Corp.