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Concentration of Enteroviruses on Membrane Filters

World Health Organization International Reference Centre for Enteroviruses, Department of Virology and Epidemiology, Baylor University College of Medicine, Houston, Texas 77025

ABSTRACT

Enteroviruses can be made to adsorb or to pass through membrane filters by manipulation of the suspending medium. Salts facilitate virus adsorption, but membrane-coating components (MCC) interfere. Because cells release MCC into the culture medium during viral growth, MCC must be removed before virus can be adsorbed to membranes. Adsorbed virus can be eluted with diluents containing MCC (cell extracts or serum) or agents that reduce surface tension (sodium lauryl sulfate). By membrane adsorption and elution, enteroviruses can be readily concentrated and quantitatively recovered from crude virus harvests.

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