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J Virol. 1967 April; 1(2): 294-301
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Herpes Simplex Virus Products in Productive and Abortive Infection I. Stabilization with Formaldehyde and Preliminary Analyses by Isopycnic Centrifugation in CsCl

Susan B. Spring and Bernard Roizman

Department of Microbiology, The University of Chicago, Chicago, Illinois 60637

ABSTRACT

Lysates of HEp-2 cells productively infected with herpes simplex virus yielded two bands on isopycnic centrifugation in CsCl gradients, ranging from 1.2 to 1.6 g/cm3. One band, designated {alpha}, had a mean buoyant density of 1.27 g/cm3 and contained herpes virions. Band ß had a mean density of 1.305 g/cm3 and contained primarily complement-fixing viral antigens and little or no viral deoxyribonucleic acid (DNA). The products banding in the {alpha} and ß bands were unstable; fivefold or higher amounts were recovered by treating the cell extract with formaldehyde prior to centrifugation. Formaldehyde treatment increased the buoyant density of viral products in both the {alpha} and ß bands by about 0.015 g/cm3. In addition, it stabilized hitherto inapparent products, forming a broad band {gamma} with a density range of 1.37 to 1.45 g/cm3. The material in the {gamma} band was heterogeneous; it contained viral DNA, cellular DNA, and viral antigen. Formalinized lysates of DK cells abortively infected with herpes simplex virus yielded a ß band undifferentiated from that formed by extracts of productively infected cells. The {gamma} band was less dense and narrower. The {alpha} band was entirely missing.

J Virol. 1967 April; 1(2): 294-301
Copyright © 1967 American Society for Microbiology. All Rights Reserved.





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Copyright © 1967 by the American Society for Microbiology. All rights reserved.