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J Virol. 1967 February; 1(1): 64-75
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Replication of Bacteriophage Ribonucleic Acid: Some Physical Properties of Single-stranded, Double-stranded, and Branched Viral Ribonucleic Acid

The Public Health Research Institute of The City of New York, Inc., New York, New York

ABSTRACT

Replicative intermediate (RI) is considered to be the double-stranded ribo-nucleic acid (RNA) template for synthesis of viral RNA, with bound nascent single-stranded viral RNA. A theoretical description of RI is based on the analysis of a steady state of biopolymerization on a template which determines not only nucleotide sequence, but also chain length. The hydrodynamic properties of RI isolated from Escherichia coli infected with bacteriophage R17 are compared with those of RNA isolated from R17 (single-stranded RNA) and of replicative form (RF) isolated from E. coli infected with R17. RF is double-stranded RNA template without any single-stranded component. Whereas S for R17 RNA is a function of the ionic strength (/2) of the solvent, S is almost invariant with /2 for RF. By contrast for RI lies between the sedimentation constants for R17 RNA and RF and varies with /2 as does R17 RNA. The weight distribution of S for RI demonstrates the heterogeneity of this material, and the variation in the weight distribution with ionic strength demonstrates the duality of structure in RI. Using [], the W for RI is estimated to be 2.6 x 10⁶ daltons, as compared with the theoretical value of 2.9 x 10⁶ daltons.

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