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Reovirus-directed Ribonucleic Acid Synthesis in Infected L Cells ¹

^a Laboratory of Biology of Viruses, National Institute of Allergy and Infectious Diseases, Bethesda, Maryland

ABSTRACT

Reovirus replication in L-929 mouse fibroblasts was unaffected by 0.5 µg of actinomycin per ml, a concentration which inhibited cell ribonucleic acid (RNA) synthesis by more than 90%. Under these conditions of selective inhibition, the formation of both single-stranded and double-stranded virus-specific RNA was detected beginning at 6 hr after infection. The purified double-stranded RNA was similar in size and base composition to virus RNA and presumably was incorporated into mature virus. The single-stranded RNA formed ribonuclease-resistant duplexes when annealed with denatured virus RNA but did not self-anneal, thus indicating that it includes copies of only one strand of the duplex. The single-stranded RNA was polyribosome-associated and may function as the virus messenger RNA. Production of both types of virus-induced RNA required protein synthesis 6 to 9 hr after infection. At later times in the infectious cycle, only double-stranded RNA synthesis was dependent on continued protein formation.

² World Health Organization Fellow. Permanent address: Institute of Virology, Czechoslovak Academy of Sciences, Bratislava, Czechoslovakia.

¹ A portion of these results was presented at the Symposium on the Molecular Biology of Viruses, University of Alberta, Edmonton, Canada, 29 June 1966.