This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Reprints and Permissions Copyright Information Books from ASM Press MicrobeWorld Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Hummeler, K. Articles by Wiktor, T. J. Search for Related Content PubMed PubMed Citation Articles by Hummeler, K. Articles by Wiktor, T. J.

 Previous Article  |  Next Article 

J Virol. 1967 February; 1(1): 152-170
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Structure and Development of Rabies Virus in Tissue Culture

^a The Children's Hospital of Philadelphia, the Department of Pediatrics, University of Pennsylvania Medical School, and The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania

ABSTRACT

Structure and development of two fixed rabies virus strains in baby hamster kidney cells (BHK/21) were investigated by electron microscopy. The morphological development was correlated with fluorescent-antibody staining and infectivity titration. The uptake of virus was enhanced by addition of diethylaminoethyl dextran, and structural changes became apparent in the cytoplasm 8 to 9 hr after infection, when fluorescent-antibody staining was first discernible. These changes consisted of matrices containing fibers replacing normal cytoplasmic structures. Virus particles appeared at the edges of these matrices and inside them at 24 to 48 hr. This corresponded to significant rises in intracellular infectious virus. Formation of virus particles by budding from cell membranes was seen at 72 hr. Further incubation of the infected cells resulted in synthesis of bizarre structural elements. The complete virus particle was bullet-shaped with an average size of 180 by 75 mµ. It consisted of an inner core of filamentous material surrounded by two membranes of different densities. The surface showed a honeycomb arrangement with surface protrusions 60 to 70 A long having a knoblike structure at their distal end. These surface protrusions were absent at the flat end of the virus particle.

¹ Recipient of Public Health Service award K3-HD-22708.

This article has been cited by other articles:

• Solon, J., Gareil, O., Bassereau, P., Gaudin, Y. (2005). Membrane deformations induced by the matrix protein of vesicular stomatitis virus in a minimal system. J. Gen. Virol. 86: 3357-3363 [Abstract] [Full Text]  
• Tuffereau, C., Benejean, J., Alfonso, A.-M. R., Flamand, A., Fishman, M. C. (1998). Neuronal Cell Surface Molecules Mediate Specific Binding to Rabies Virus Glycoprotein Expressed by a Recombinant Baculovirus on the Surfaces of Lepidopteran Cells. J. Virol. 72: 1085-1091 [Abstract] [Full Text]