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J Virol. 1967 February; 1(1): 145-151
Copyright © 1967 American Society for Microbiology. All Rights Reserved.

Effect of Polyions on the Infectivity of Rabies Virus in Tissue Culture: Construction of a Single-Cycle Growth Curve

M. M. Kaplan1, T. J. Wiktor, R. F. Maes2, J. B. Campbell and H. Koprowski

a The Wistar Institute of Anatomy and Biology, Philadelphia, Pennsylvania

ABSTRACT

The infectivity of fixed rabies virus in a number of cell lines has been shown to be markedly enhanced by the addition of protamine or diethylaminoethyl dextran to the virus inoculum. The polycations appear to exert their influence at a very early stage (adsorption or penetration or both) of virus-cell interaction. Immune globulin blocked infection completely when added up to 5 min after exposure and almost completely when added 5 to 15 min after infection. Antibody had no effect on adsorption and penetration when added to the inoculum 30 min or more after cells were exposed to the virus. Irradiation of BHK/21 cell monolayers with ultraviolet light increased their sensitivity to rabies virus. The events occurring after synchronous infection of cells in both irradiated and nonirradiated cell monolayers were followed by means of fluorescent-antibody staining and by intracerebral titration in mice. Virus-specific fluorescent antigen first appeared between 8 and 9 hr after infection, and in irradiated cultures there was a further lag period of 3 hr before infectious virus was produced intracellularly. Virus was first detected in the medium 12 to 15 hr after infection, and maximal yield of infectious virus was observed 48 hr after exposure. In nonirradiated cultures, formation of infectious virus was delayed, and the final yield of virus was also reduced.

FOOTNOTES

1 Present address: Veterinary Public Health, World Health Organization, Geneva, Switzerland.

2 Present address: St. Jude Children's Hospital, Memphis, Tenn.

J Virol. 1967 February; 1(1): 145-151
Copyright © 1967 American Society for Microbiology. All Rights Reserved.





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Copyright © 1967 by the American Society for Microbiology. All rights reserved.